Abstract:The metal uptake capacity of a bacterial strain (namely Z-2) which was isolated from both activated sludge and heavy metals contaminated soil adjacent to an electroplating plant was investigated. The biosorption rate and nickle tolerance of Z-2 were significantly improved by acclimated by high metal concentration solution. The optimal parameters for Z-2 were pH at 6.0, temperature at 30～40℃, 8 hours’ sorption time, 100 mg?L-1 of zinc and 50 mg?L-1 of nickel concentration, and a biomass of 3 g?L-1 with 3 days’ culturing, with these these Ni2+ removal with corresponding sorption rate of 30.67 % could be reached. The absorption models matched the Langmuir Isotherm Adsorption Equation.